Reporter

Part:BBa_K1878001:Design

Designed by: John Luke Garcia   Group: iGEM16_Rice   (2016-10-14)


iRFP713 (the fluorescent protein formerly known as "iRFP")


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
    Illegal XhoI site found at 346
    Illegal XhoI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PstI site and EcoRI site within the reporter has been removed. There is a Bbs1 site within the coding sequence so be wary if using that enzyme for Golden Gate DNA assembly.



Source

iRFP713 is a Rhodopseudomonas palustris bacterial phytochrome photoreceptor.


References